Real-Time PCR
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Real-time PCR Kits
Takara Bio - Premix Ex Taq™
Master mix for probe-based real-time PCR
The Premix Ex Taq master mix is ideal for high-speed PCR, allows accurate target quantification, and enables highly reproducible and realiable PCR analyses.
- Rapid and accurate PCR product detection
- Quantitative gene expression analysis using real-time PCR (qPCR)
- 2X master mix enables easy pipetting
- Optimized buffer increases amplification efficiency, highly sensitive probe-based qPCR assays, and 5' nuclease assays
- Included Tli RNase H minimizes PCR inhibition by removing residual mRNA in input cDNA
Real-time PCR Kits
Takara Bio - TB Green® Premix Ex Taq™ (Tli RNase H Plus)
qPCR with TB Green detection
Designed for high-speed, high-sensitivity qPCR. The premix limits PCR inhibition from residual mRNA and provides superior specificity and fidelity compared to conventional Taq DNA Polymerase.
- Lower Cq values as a result of fast extension rates
- Use with broad range of amplicon sizes, even over 500 bp
- Convenient 2X master mix is excellent for high-speed qPCR
- High sensitivity: detects fewer than 100 copies
- Hot-start PCR enzyme enables high efficiency and sensitivity in real-time PCR (qPCR)
- Compatible with all commonly used real-time PCR (qPCR) instruments
- Minimizes qPCR inhibition due to residual mRNA in input cDNA
- Includes TB Green for intercalator-based qPCR
Real-time PCR Kits
Takara Bio - One-Step TB Green® PrimeScript™ RT-PCR Kit II (Perfect Real Time)
qPCR with TB Green detection
For intercalator-based, one-step RT-qPCR. Includes TB Green detection, extension of longer templates and GC-rich RNA, optimized buffer for high specificity, and high-efficiency hot-start PCR enzyme.
- Quantitative real-time PCR enables rapid and accurate analysis of RNA from viral, rare, or precious samples
- The One-Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time) buffer system is optimized for quantitative real-time PCR, allowing efficient amplification and highly sensitive detection
- One-Step TB Green RT-PCR Buffer IV is a 2X TB Green premix; the premix formulation simplifies reaction mixture preparation
Real-time PCR Kits
Takara Bio - One Step PrimeScript™ III RT-PCR Kit
qPCR with probe detection
A master mix for performing one-step real-time, probe-based RT-qPCR in a single tube using a fast, easy protocol.
- Fast, easy one-step RT-qPCR in a single tube using probe-based detection
- Efficient, specific cDNA synthesis and amplification, also from complex RNA templates
- Suitable for a wide variety of samples from different sources, including crude RNA extracts
- Quick and simple protocol reduces the risk of contamination
Reverse Transcription Prior to qPCR
Takara Bio - PrimeScript™ RT Master Mix (Perfect Real Time)
Convenient master mix for real-time PCR
Allows fast, efficient cDNA template synthesis for real-time PCR. The PrimeScript RT Master Mix enables full-length cDNA synthesis for real-time PCR in just 15 minutes.
- First-strand cDNA synthesis for real-time RT-PCR (qPCR)
- Two-step real-time RT-PCR
- Reverse transcription in just 15 minutes
- Master mix format reduces pipetting steps and the risk of contamination
- Works efficiently with GC-rich and/or higher-structured RNA templates
- Synthesizes full-length cDNA with high accuracy and at high yield prior to qPCR analysis
Multiplex Gene Expression Assay
eBioscience - QuantiGene™ Plex
Multiplexed gene expression quantitation
Accurate and precise RNA quantitation of up to 80 genes in a single well.
- Multiplex and customizable RNA or DNA measurement using a Luminex® instrument
- No need for RNA purification or target amplification - just lyse and run
- Works with difficult sample types, including FFPE, and directly with blood
Preamp and Reverse Transcription Master Mixes
Fluidigm - Master Mixes
Preamp and Reverse Transcription Master Mixes
Fluidigm delivers optimized Preamp and Reverse Transcription Master Mixes for the Biomark and Biomark HD platforms. Preamplification allows for multiplex amplification of up to 96 targets. A pool of primers is prepared from the same gene expression assays to be used for qPCR. By using the real-time qPCR assays in the preamplification reaction, only the targets of interest are amplified. A limited number of cycles is used and the cDNA is amplified without significant bias.
- Optimized for small volumes
- Optimized for TaqMan Gene Expression Assays
Integrated Fluidic Circuits (IFCs)
Fluidigm - Biomark HD IFCs
Make up to 9.216 datapoints in one run
Enhance your productivity with high-performance solutions for robust processing of samples in nanoscale reactions, enabling quick and reliable gene expression, copy count, and digital PCR. The revolutionary integrated fluidic circuits (IFCs) enable automated molecular biology that significantly minimizes pipetting steps, hands-on time, and reagent consumption while maximizing efficiency, cost savings, and robust data production.
- Find the format that covers your project from 12-192 samples combined with 12-192 assays
- Sample.Assays:
- 192.24
- 96.96
- 48.48
- 24.192
- 12.12*6